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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
01/10/2019 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A. |
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Afiliación : |
FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036. |
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DOI : |
10.1017/S1431927619000692 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. |
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Contenido : |
Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. |
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Palabras claves : |
BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02045naa a2200229 a 4500
001 1060255
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S1431927619000692$2DOI
100 1 $aBÁEZ, F.
245 $aUltrastructural imaging analysis of the zona pellucida surface in bovine oocytes.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019.
520 $aAbstract: The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes (COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development.
653 $aBOVINE
653 $aIN VITRO MATURATION
653 $aPLATAFORMA SALUD ANIMAL
653 $aULTRASTRUCTURE
653 $aZONA PELLUCIDA
700 1 $aCAMARGO, A.A.
700 1 $aGASTAL, G.D.A.
773 $tMicroscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
30/05/2019 |
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Actualizado : |
22/04/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CAZZULI, F.; DE BARBIERI, I.; JAURENA, M.; SILVEIRA, C.; MONTOSSI, F. |
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Afiliación : |
FIORELLA CARLA CAZZULI ALBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN ALEJANDRO JAURENA BARRIOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA INES SILVEIRA ROJAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Restricted oats grazing and its frequency of allocation affects calves´ performance on native grasslands. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Arch. Zootec., 2019, v. 68, no. 262, p. 220-227.OPEN ACCESS. DOI: https://doi.org/10.21071/az.v68i262.4140 |
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ISSN : |
18854494 |
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DOI : |
10.21071/az.v68i262.4140 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido/Received: 25.01.2018; Aceptado/Accepted: 01.09.2018; On-line: 07.04.2019. Correspondencia a los autores/Contact e-mail:
fcazzuli@inia.org.uy ACKNOWLEDGMENTS: This study was financially supported by INIA Uruguay. The authors would like to thank staff of “Glencoe” Research Station for their invaluable assistance during the present study. |
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Contenido : |
SUMMARY: Nutritional constraints of rearing calves on native grasslands during their first winter is a common problem in Uruguay due to insufficient quality and quantity of forage. Winter supplementation on beef cattle grazing native grasslands using grains or their by-products has been studied before, but little attention has been given to restricting the access of animals to an adjacent enhanced forage basis, such as oats forage. Thirty-six castrated Hereford calves grazing native grasslands during their first winter were randomly allotted to one of the following treatments: (a) Everyday (E), with access to an adjacent oats forage crop for 4 hours a day (28 hours/week); (b) Monday-through-Friday (MthF), with access to the same crop for 5 hours 36 min per day (28 hours/week); (c) Control (C), where animals did not have any access to oats. The experiment was separated in two Phases: Phase I where treatments were applied (winter), and Phase II, after the treatments were applied (spring), to evaluate eventual carry-over effects. Restricted grazing on oats affected herbage mass and sward height of native grasslands in both experimental phases (P<0.01), being greater for E than C and MthF. Average daily gain (ADG) was affected in Phase I (P<0.01) by time-restricted grazing access to oats, being greater for E (745 g), followed by MthF (645 g) and then by C (377 g). During Phase II, ADG was greatest (P<0.05) for E (673 g), while MthF and C presented no differences between them (508 g, on average). When considering the whole evaluation period (Phases I & II), animals from E and MthF groups gained 59 and 28% more respectively, than animals from the control group, being all significantly different between each other (P<0.01). The use of deferred native grasslands as a nutritive basis combined with a restricted access to an oats forage crop proved to be an interesting technological tool to improve calves´ performance during winter. Positive feedbacks between pasture supplementation and native grasslands were identified, which could contribute to a better management of this forage basis in extensive production systems. // RESUMEN: El pastoreo restringido de avena y su frecuencia de asignación afecta el desempeño de terneros pastoreando campo natural: Las restricciones nutricionales de los terneros de recría sobre campo natural durante su primer invierno es un problema común en Uruguay, dada la insuficiente calidad y cantidad del forraje. La suplementación invernal del ganado de carne utilizando granos o sus subproductos y pastoreando sobre pasturas nativas, ha sido estudiada previamente, pero muy poca atención se le ha dado a el acceso restringido de animales a una base forrajera mejorada adyacente, como lo puede ser un verdeo de avena. Treinta y seis terneros machos castrados pastoreando sobre campo natural durante su primer invierno fueron asignados al azar a uno de los siguientes tratamientos: (a) Diario (D) con acceso a una parcela adyacente de avena por 4 horas al día (28 horas/semana); (b) de Lunes a Viernes (LaV) con acceso al mismo verdeo por 5 horas 36 minutos por día (28 horas/semana); (c) Testigo (T), en el que los animales no tenían acceso alguno al verdeo de avena. El experimento fue dividido en dos fases: la fase I en donde los tratamientos fueron aplicados (invierno) y la fase II, luego que los mismos fueran aplicados (primavera), a los efectos de evaluar un eventual arrastre de efectos. El acceso restringido a la avena afectó la disponibilidad y altura del forraje del campo natural en ambas fases evaluadas (P<0,01), siendo mayor para D que T y LaV. Las ganancias medias diarias (gmd) fueron afectadas durante la fase I (P<0,01) por el acceso restringido a la avena, siendo mayor para D (745 g), seguido por LaV (645 g) y luego por T (377 g). Durante la fase II, las gmd fueron mayores (P<0,05) para D (673 g), mientras que LaV y T no presentaron diferencias entre ellos (508 g en promedio). Al considerar la totalidad del período de evaluación (fases I y II), los animales correspondientes a D y LaV ganaron 59 y 28 % más respectivamente, que los animales correspondientes a T, siendo todos significativamente diferentes entre sí (P<0,01). El uso de campo natural diferido como base forrajera, combinado con un acceso restringido al forraje de avena demostró ser una tecnología válida para mejorar el desempeño de los terneros durante el invierno. Se identificaron retroalimentaciones positivas entre la suplementación con forraje y el campo natural, lo cual podría contribuir a un mejor manejo de esta base forrajera en los sistemas extensivos ganaderos. MenosSUMMARY: Nutritional constraints of rearing calves on native grasslands during their first winter is a common problem in Uruguay due to insufficient quality and quantity of forage. Winter supplementation on beef cattle grazing native grasslands using grains or their by-products has been studied before, but little attention has been given to restricting the access of animals to an adjacent enhanced forage basis, such as oats forage. Thirty-six castrated Hereford calves grazing native grasslands during their first winter were randomly allotted to one of the following treatments: (a) Everyday (E), with access to an adjacent oats forage crop for 4 hours a day (28 hours/week); (b) Monday-through-Friday (MthF), with access to the same crop for 5 hours 36 min per day (28 hours/week); (c) Control (C), where animals did not have any access to oats. The experiment was separated in two Phases: Phase I where treatments were applied (winter), and Phase II, after the treatments were applied (spring), to evaluate eventual carry-over effects. Restricted grazing on oats affected herbage mass and sward height of native grasslands in both experimental phases (P<0.01), being greater for E than C and MthF. Average daily gain (ADG) was affected in Phase I (P<0.01) by time-restricted grazing access to oats, being greater for E (745 g), followed by MthF (645 g) and then by C (377 g). During Phase II, ADG was greatest (P<0.05) for E (673 g), while MthF and C presented no differences between them (50... Presentar Todo |
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Palabras claves : |
CAMPO NATURAL; FORRAJE COMO SUPLEMENTO; NATURAL MEADOW; RECRÍA RAZA CARNICERAS; SUPLEMENTACIÓN INFRECUENTE; VERDEO INVERNAL. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12709/1/Montossi-2019-Archivos.pdf
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Marc : |
LEADER 05895naa a2200277 a 4500
001 1059791
005 2020-04-22
008 2019 bl uuuu u00u1 u #d
022 $a18854494
024 7 $a10.21071/az.v68i262.4140$2DOI
100 1 $aCAZZULI, F.
245 $aRestricted oats grazing and its frequency of allocation affects calves´ performance on native grasslands.$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido/Received: 25.01.2018; Aceptado/Accepted: 01.09.2018; On-line: 07.04.2019. Correspondencia a los autores/Contact e-mail: fcazzuli@inia.org.uy ACKNOWLEDGMENTS: This study was financially supported by INIA Uruguay. The authors would like to thank staff of “Glencoe” Research Station for their invaluable assistance during the present study.
520 $aSUMMARY: Nutritional constraints of rearing calves on native grasslands during their first winter is a common problem in Uruguay due to insufficient quality and quantity of forage. Winter supplementation on beef cattle grazing native grasslands using grains or their by-products has been studied before, but little attention has been given to restricting the access of animals to an adjacent enhanced forage basis, such as oats forage. Thirty-six castrated Hereford calves grazing native grasslands during their first winter were randomly allotted to one of the following treatments: (a) Everyday (E), with access to an adjacent oats forage crop for 4 hours a day (28 hours/week); (b) Monday-through-Friday (MthF), with access to the same crop for 5 hours 36 min per day (28 hours/week); (c) Control (C), where animals did not have any access to oats. The experiment was separated in two Phases: Phase I where treatments were applied (winter), and Phase II, after the treatments were applied (spring), to evaluate eventual carry-over effects. Restricted grazing on oats affected herbage mass and sward height of native grasslands in both experimental phases (P<0.01), being greater for E than C and MthF. Average daily gain (ADG) was affected in Phase I (P<0.01) by time-restricted grazing access to oats, being greater for E (745 g), followed by MthF (645 g) and then by C (377 g). During Phase II, ADG was greatest (P<0.05) for E (673 g), while MthF and C presented no differences between them (508 g, on average). When considering the whole evaluation period (Phases I & II), animals from E and MthF groups gained 59 and 28% more respectively, than animals from the control group, being all significantly different between each other (P<0.01). The use of deferred native grasslands as a nutritive basis combined with a restricted access to an oats forage crop proved to be an interesting technological tool to improve calves´ performance during winter. Positive feedbacks between pasture supplementation and native grasslands were identified, which could contribute to a better management of this forage basis in extensive production systems. // RESUMEN: El pastoreo restringido de avena y su frecuencia de asignación afecta el desempeño de terneros pastoreando campo natural: Las restricciones nutricionales de los terneros de recría sobre campo natural durante su primer invierno es un problema común en Uruguay, dada la insuficiente calidad y cantidad del forraje. La suplementación invernal del ganado de carne utilizando granos o sus subproductos y pastoreando sobre pasturas nativas, ha sido estudiada previamente, pero muy poca atención se le ha dado a el acceso restringido de animales a una base forrajera mejorada adyacente, como lo puede ser un verdeo de avena. Treinta y seis terneros machos castrados pastoreando sobre campo natural durante su primer invierno fueron asignados al azar a uno de los siguientes tratamientos: (a) Diario (D) con acceso a una parcela adyacente de avena por 4 horas al día (28 horas/semana); (b) de Lunes a Viernes (LaV) con acceso al mismo verdeo por 5 horas 36 minutos por día (28 horas/semana); (c) Testigo (T), en el que los animales no tenían acceso alguno al verdeo de avena. El experimento fue dividido en dos fases: la fase I en donde los tratamientos fueron aplicados (invierno) y la fase II, luego que los mismos fueran aplicados (primavera), a los efectos de evaluar un eventual arrastre de efectos. El acceso restringido a la avena afectó la disponibilidad y altura del forraje del campo natural en ambas fases evaluadas (P<0,01), siendo mayor para D que T y LaV. Las ganancias medias diarias (gmd) fueron afectadas durante la fase I (P<0,01) por el acceso restringido a la avena, siendo mayor para D (745 g), seguido por LaV (645 g) y luego por T (377 g). Durante la fase II, las gmd fueron mayores (P<0,05) para D (673 g), mientras que LaV y T no presentaron diferencias entre ellos (508 g en promedio). Al considerar la totalidad del período de evaluación (fases I y II), los animales correspondientes a D y LaV ganaron 59 y 28 % más respectivamente, que los animales correspondientes a T, siendo todos significativamente diferentes entre sí (P<0,01). El uso de campo natural diferido como base forrajera, combinado con un acceso restringido al forraje de avena demostró ser una tecnología válida para mejorar el desempeño de los terneros durante el invierno. Se identificaron retroalimentaciones positivas entre la suplementación con forraje y el campo natural, lo cual podría contribuir a un mejor manejo de esta base forrajera en los sistemas extensivos ganaderos.
653 $aCAMPO NATURAL
653 $aFORRAJE COMO SUPLEMENTO
653 $aNATURAL MEADOW
653 $aRECRÍA RAZA CARNICERAS
653 $aSUPLEMENTACIÓN INFRECUENTE
653 $aVERDEO INVERNAL
700 1 $aDE BARBIERI, I.
700 1 $aJAURENA, M.
700 1 $aSILVEIRA, C.
700 1 $aMONTOSSI, F.
773 $tArch. Zootec., 2019$gv. 68, no. 262, p. 220-227.OPEN ACCESS. DOI: https://doi.org/10.21071/az.v68i262.4140
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